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<h1>Multiple Forms of Pectinesterase are Present in Tomato and Other Fruit</h1>
<h2>Pectinesterase Isoforms</h2>
<p>There are now a large number of tomato PE N-terminal protein sequences available in
the literature. These are shown in the table below:</p>
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<th colspan="4" bgcolor="#CCCCCC"><font color="#FFFFFF">Table 1. Tomato PE N-terminal
sequences</font></th>
</tr>
<tr>
<td bgcolor="#CCCCCC"><b>Sequence</b></td>
<td bgcolor="#CCCCCC"><b>Name</b></td>
<td bgcolor="#CCCCCC"><b>Cultivar</b></td>
<td bgcolor="#CCCCCC"><b>Reference</b></td>
</tr>
<tr>
<td bgcolor="#DDDDDD">IIANAVVAQDGTGDYQYLAEAVAAA</td>
<td bgcolor="#DDDDDD">?</td>
<td bgcolor="#DDDDDD">Immuna</td>
<td bgcolor="#DDDDDD">Markovic & Jörnvall (1986)</td>
</tr>
<tr>
<td bgcolor="#FFFFFF">IIANAVVAQDGTGDYQLTAEAVAAA</td>
<td bgcolor="#FFFFFF">PE-A</td>
<td bgcolor="#FFFFFF">Ailsa Craig</td>
<td bgcolor="#FFFFFF">Warrilow et al. (1994)</td>
</tr>
<tr>
<td bgcolor="#DDDDDD">LSXGQVESGLAPXLPYLQGKGPLGG</td>
<td bgcolor="#DDDDDD">PE-C</td>
<td bgcolor="#DDDDDD">Ailsa Craig</td>
<td bgcolor="#DDDDDD">Warrilow et al. (1994)</td>
</tr>
<tr>
<td bgcolor="#FFFFFF">EDPYRYFDW</td>
<td bgcolor="#FFFFFF">PE3</td>
<td bgcolor="#FFFFFF">Ailsa Craig</td>
<td bgcolor="#FFFFFF">Zhang, 1994</td>
</tr>
<tr>
<td bgcolor="#DDDDDD">IIANAVVAQDGTGKYRTLAEAV</td>
<td bgcolor="#DDDDDD">PEIII</td>
<td bgcolor="#DDDDDD">Short Red</td>
<td bgcolor="#DDDDDD">Pressey & Woods, (1992)</td>
</tr>
<tr>
<td bgcolor="#FFFFFF">IIANAVVAQDGTGDYQTLAEAV</td>
<td bgcolor="#FFFFFF">PEIV</td>
<td bgcolor="#FFFFFF">Better Boy</td>
<td bgcolor="#FFFFFF">Pressey & Woods (1992)</td>
</tr>
<tr>
<td bgcolor="#DDDDDD">IGANAVVAKDGTGKYRTLAEAVAAA</td>
<td bgcolor="#DDDDDD">pPE1</td>
<td bgcolor="#DDDDDD">Ailsa Craig</td>
<td bgcolor="#DDDDDD">Ray et al. (1988)</td>
</tr>
<tr>
<td bgcolor="#FFFFFF">IIANAVVAQDGTGDYQTLAEAVAAA</td>
<td bgcolor="#FFFFFF">pB8</td>
<td bgcolor="#FFFFFF">Ailsa Craig</td>
<td bgcolor="#FFFFFF">Hall et al. (1994)</td>
</tr>
<tr>
<td bgcolor="#DDDDDD">IGANAVVAKDGTGKYRTLAEAVAAA</td>
<td bgcolor="#DDDDDD">pB16</td>
<td bgcolor="#DDDDDD">Ailsa Craig</td>
<td bgcolor="#DDDDDD">Hall et al. (1994)</td>
</tr>
</table>
</center>
<p>As can be seen from the table, virtually all of the sequences are very
similar to the first PE protein sequenced by Markovic & Jörnvall. This corresponds to
the main isoform known as PE2. Some of the minor differences between the N-terminal
sequences were thought to be due to differences between the tomato varieties studied.
However, the pB8 & pB16 sequences were both derived from distinct cDNA clones of the
cultivar Ailsa Craig, so perhaps there are "sub-isoforms" of PE2. To confuse
matters further, the sequences of PE-C (PE1) and PE3 share no homology to the rest. Our
sequencing of the putative cDNA for PE3 shows no homology to PE2, so these could be PEs
unrelated to PE2.</p>
<h2>Further Reading</h2>
<ul>
<li><a href="PE_default.asp">Title Page</a></li>
<li><a href="PE_introduction.asp">Introduction</a></li>
<li><a href="PE_assaydev.asp">Assay development</a></li>
<li><a href="PE_totalpe.asp">Total Pectinesterase activity in fruit</a></li>
<li><a href="PE_tomato.asp">Pectinesterase in Tomato</a></li>
<li><a href="PE_strawberry.asp">Pectinesterase in Strawberry</a></li>
<li><a href="PE_citrus.asp">Pectinesterase in citrus fruits</a></li>
<li><a href="PE_grape.asp">Pectinesterase in Grapes</a></li>
<li><a href="PE_summary.asp">Summary & Future work</a></li>
<li><a href="PE_references.asp">References</a></li>
</ul>
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